Wednesday, July 13, 2011

Agrobacterium tumefacien regeneration process?

I don't think the addtion of antibiotic is to kill the bacteria. Since the disarmed Ti plasmid is engineered along with pBR322 which has got Ampicilin resistant gene, which is a marker for identification. So they will add ampilicin to the select or identify the engineered organism. It also be selected using opaline presence or absence.

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